Vitamin E
It’s interesting how feed/supplement companies have made horse owners think that they need to supplement all the vitamins. Research has shown that it’s generally unnecessary to supplement vitamins for horses on high forage diets. Of course, there may be exceptions but they are literally exceptions to the rule. Many of the vitamins are manufactured by the horse (via gut microbial fermentation) or are in the pasture and hay that horses eat. The main exception is vitamin E, especially for horses that are fed significant quantities of processed feed/hay or are in work.
Like selenium, vitamin E is an important antioxidant, especially in the neuromuscular and immune systems. Antioxidants protect the body against damage from reactive molecules, known as oxygen free radicals. These are generated in the body as a result of metabolism and cause cell membrane damage. It’s like the chemical reaction that creates rust on a bicycle or turns the surface of a cut apple brown – oxidation.
For horses in work, a vitamin E dietary shortfall can cause muscular problems such as poor performance, poor recovery, muscle weakness and soreness. It can even resemble ‘tying up’. Horses working at high levels produce large amounts of reactive molecules resulting in cell damage. Sufficient levels of vitamin E has a positive effect on muscle integrity in hard working horses, helping to neutralise free radicals which damage and weaken muscle tissue.
A number of specific conditions are associated with vitamin E deficiency including Equine Motor Neuron Disease (EMND), Neuroaxonal Dystrophy (equine NAD)/Degenerative Equine Myeloencephalopathy (EDM) and Vitamin E Deficient Myopathy.
Many studies have shown that the highest levels of vitamin E is in fresh grass. Ohio State University found that levels varied from 25-190 IU/kg (average, 110 IU/kg). IU = International units, the number of IU is a measure of potency. Schingoethe et al found levels ranging from 106 to 309 IU/kg (average, 219 IU/kg). Ballet et al found an average of 240 IU/kg and the older or more mature the stand of grass, the lower the vitamin E level. For some situations, this could mean up to 2000 IU or more per day, exceeding the NRC recommendations. From this we know that generally horses in no work where the intake is entirely or mainly fresh grass, especially actively growing grass don’t require additional vitamin E supplementation. For horses on mature stands of grass, there is no harm in feeding supplementary natural vitamin E, a reasonable amount is 500 IU/day.
Vitamin E content is lowest in hay with declining concentrations due to processing and storage.
If your horse is in work, or doesn’t have the luxury of a high intake of fresh grass, then yes, vitamin E supplementation is beneficial. Many studies have shown horses benefit from vitamin E supplementation – some examples are below in the further reading section.
Vitamin E in mineral/vitamin mixes and feed products
Why do none of the Balanced Equine mineral/vitamin mixes not contain vitamin E? Not even HoofXtra? Vitamin E added to multi ingredient supplements or feeds is oxidised very quickly so ideally, keep vitamin E separate rather than wasting money on an expensive vitamin. Unfortunately vitamin E is heat sensitive so even if a commercial feed or supplement lists it as an ingredient it can’t be entirely counted. Vitamin E can react with inorganic minerals, especially iron. If there is any organic carrier in the mix, it will also act as a natural antioxidant as fats oxidise or bacterial or fungal growth occurs.
Vitamin E is best added separately when making up a feed for your horse.
An excellent source is human liquid vitamin E capsules. Technically you could use either synthetic or natural as 400 natural IU should be equivalent to 400 synthetic IU, though there is some evidence that natural vitamin E is more bioavailable than synthetic vitamin E. In the long term, they may be the same. Nevertheless, the recommendation is to feed natural vitamin E (d-alpha tocopherol) rather than synthetic vitamin E (labelled ‘dl’ rather than ‘d’).
As a guideline, if you are recommended to feed 500 IU natural vitamin E, increase it to 625 IU for the synthetic version.
Not sure which Balanced Equine mix to feed with your vitamin E capsules/liquid product? Read Which Mix.
How to feed vitamin E?
Throw the capsules straight into a feed bucket, many horses happily eat the capsules but if a horse is a bit picky, the next best option is to soak the capsules in a little bit of tepid water to soften the capsules and then add to the feed or cut the capsules up first with scissors.
Vitamin E capsules can be purchased online or pharmacies or supermarkets. Look for a brand of natural vitamin E capsules that has some oil mixed with the vitamin E rather than just glycerin/gelatin on their own. Can be any oil, safflower oil and soybean oil are commonly used. You may have to directly ask the manufacturer. This matters as uptake into the bloodstream is more likely if vitamin E is coated with oil since vitamin E is fat soluble. It isn’t enough to just have some fat or oil in the feed bucket, it will be diluted too much. If you decide to use a powdered (not liquid), mix or coat it with a little oil. Without the oil, it is far less likely to pass through the intestinal wall and be excreted instead in the manure.
Vitamin E in the intestine has to “find” dietary fats to be absorbed. Vitamin E inside grass is already associated with fats or phospholipids when eaten. Vitamin E in the intestine has to ‘find’ dietary fat or oil to be absorbed well.
Vitamin E should be used up within six months or less. Moisture and air (oxygen) are damaging over time as potency decreases with oxidation. Keep out of direct sunlight, heat and keep the container tightly closed.
Further reading
Links may change over time. If a link doesn’t work, search the title in your search engine.
Ballet N, Robert JC and Williams PE
Chapter 19: Vitamins in Forages in Givens DI et al Forage Evaluation in Ruminant Nutrition
https://www.robertorubino.eu/wp-content/uploads/2016/05/Ballet-2000-Vitamins-in-Forages.pdf
Fagan MM, Harris P, Adams A, Pazdro R, Krotky A, Call J, Duberstein KJ
Form of vitamin E supplementation affects oxidative and inflammatory response in exercising horses
https://www.sciencedirect.com/science/article/pii/S0737080620301945
Finno CJ and Valberg SJ (2012) A comparative review of vitamin E and associated equine disorders
https://onlinelibrary.wiley.com/doi/full/10.1111/j.1939-1676.2012.00994.x
Garcia EIC, Elghandour MMMY, Khusro A, Alcala-Canto Y, Tirado-González DN, Barbabosa-Pliego A, Salem AZM
Dietary supplements of vitamins E, C, and β-carotene to reduce oxidative stress in horses: An overview
https://www.sciencedirect.com/science/article/pii/S0737080622000016
Iuliano L, Micheletta F, Maranghi M, Frati G, Diczfalusy U and Violi F
Bioavailability of vitamin E as function of food intake in healthy subjects
https://www.ahajournals.org/doi/full/10.1161/hq1001.098465
Rey AI, Segura J, Arandilla E and Lopez-Bote CJ
Short- and long-term effect of oral administration of micellized natural vitamin E (D-a-tocopherol) on oxidative status in race horses under intense training
https://pubmed.ncbi.nlm.nih.gov/23296828/
Antioxidant supplementation and subsequent oxidative stress of horses during an 80-km endurance race
https://www.ncbi.nlm.nih.gov/pubmed/1497455